IGCSE · Biology 0610 · Movement into & out of cells

Diffusion: IGCSE Biology questions with answers

Free Diffusion questions for IGCSE Biology (0610), part of Movement into & out of cells. Each question has the answer and the marking points an examiner would look for.

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Diffusion practice questions

Question 1Multiple choice · 1 mark

What is diffusion?

  1. A movement of water through a partially permeable membrane
  2. B net movement of particles from high to low concentration
  3. C movement of particles from low to high concentration
  4. D movement against a concentration gradient using energy
Show answer

Answer: B

Question 2Multiple choice · 1 mark

Which term is described by this statement?
“The net movement of particles from a region of higher concentration to a region of lower concentration, as a result of their random movement.”

  1. A diffusion
  2. B oestrogen
  3. C pollination
  4. D hormone
Show answer

Answer: A

Question 3Structured · 5 marksA* challenge

Students cut agar cubes of different sizes containing an indicator and put them in acid. The colour change spreads in from the surface.

(a)

Predict which cube changes colour completely first.

[1]
(b)

Explain your answer using the idea of surface area to volume ratio.

[2]
(c)

Explain why large organisms need specialised gas exchange surfaces.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)smallest cube
the smallest cube B1
1
(b)larger SA:V, shorter distance
the smallest cube has the largest surface area to volume ratio B1
acid diffuses a shorter distance to reach the centre B1
2
(c)small SA:V, diffusion too slow
they have a small surface area to volume ratio B1
diffusion across the body surface alone would be too slow B1
2
Question 4Structured · 3 marksA* challenge

Students investigate diffusion using beetroot cubes in water at different temperatures.

(a)

Predict how temperature affects the rate at which red pigment leaves the cubes.

[1]
(b)

Explain your prediction.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)faster at higher temperature
faster at higher temperature B1
1
(b)more kinetic energy; membranes damaged
particles have more kinetic energy and move faster B1
at high temperatures the membranes are damaged/denatured B1
2
Question 5Structured · 4 marks

Oxygen moves into cells by diffusion.

(a)

Define diffusion.

[2]
(b)

State two factors that increase the rate of diffusion.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)net movement down concentration gradient by random movement
the net movement of particles from a region of higher concentration to a region of lower concentration B1
as a result of their random movement B1
2
(b)temperature; concentration gradient; surface area; shorter distance
Any 2 of: higher temperature; steeper concentration gradient; larger surface area; shorter diffusion distance B2
2
Question 6Structured · 3 marks

Gases move by diffusion in plants and animals.

(a)

Explain why carbon dioxide diffuses into a leaf during the day.

[2]
(b)

Explain why diffusion is important for single-celled organisms.

[1]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)used in photosynthesis, lower inside
carbon dioxide is used in photosynthesis B1
so its concentration inside the leaf is lower than outside B1
2
(b)exchange across surface
they obtain oxygen and remove waste across their surface by diffusion B1
1

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Original Cambridge-style practice questions written for mentorriyaz.in. These are not Cambridge past paper questions, and this site is not affiliated with or endorsed by Cambridge University Press & Assessment. Updated 2026-11-04. Paper generator · mentorriyaz.in