IGCSE · Biology 0610 · Transport

Water uptake & transpiration: IGCSE Biology questions with answers

Free Water uptake & transpiration questions for IGCSE Biology (0610), part of Transport. Each question has the answer and the marking points an examiner would look for.

Generate a full Water Uptake & Transpiration worksheet →All Transport questions

Water uptake & transpiration practice questions

Question 1Structured · 4 marksA* challenge

A potometer is used to measure the rate of water uptake of a leafy shoot.

(a)

State two conditions that increase the rate of transpiration.

[2]
(b)

Explain how an increase in humidity affects the rate of transpiration.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)higher temperature; wind; low humidity; light
Any 2 of: higher temperature; higher wind speed; lower humidity; higher light intensity B2
2
(b)less steep gradient, slower
the air is more saturated with water vapour B1
the concentration gradient is smaller, so diffusion of water vapour out of the leaf is slower B1
2
Question 2Structured · 3 marksA* challenge

In a potometer the air bubble moves 30 mm in 5 minutes. The capillary tube has a cross-sectional area of 1 mm².

(a)

Calculate the rate of water uptake in mm³ per minute.

[2]
(b)

Explain why water uptake is not exactly equal to transpiration.

[1]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)6 mm³/min
volume = 30 mm³ C1
÷ 5 = 6 mm³/min A1
2
(b)some water used in photosynthesis/kept in cells
some water is used for photosynthesis or to keep cells turgid B1
1
Question 3Structured · 6 marks

Water moves from the soil to the leaves of a plant.

(a)

Describe the pathway of water from the soil to the air.

[4]
(b)

Define transpiration.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)root hair → cortex → xylem → mesophyll → evaporates → stomata
Any 4 of: root hair cells (osmosis); across the root cortex; up the xylem; to mesophyll cells; evaporates into air spaces and diffuses out through stomata B4
4
(b)loss of water vapour from leaves
loss of water vapour from the leaves B1
by evaporation from mesophyll surfaces and diffusion through stomata B1
2
Question 4Structured · 4 marks

Transpiration has advantages for plants.

(a)

State two functions of transpiration.

[2]
(b)

Explain why plants may wilt on hot, dry, windy days.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)transport of water and minerals; cooling; turgidity
Any 2 of: transport of water and mineral ions; cooling the leaf; keeping cells turgid B2
2
(b)transpiration faster than uptake
water is lost by transpiration faster than it is absorbed by the roots B2
2
Question 5Structured · 13 marks

A student uses a potometer to measure water uptake by a leafy shoot. The air bubble moves 37.5 mm in 5 minutes. The capillary tube has a radius of 0.50 mm.

(a)

Define transpiration.

[2]
(b)

Calculate the rate of movement of the bubble in mm per minute.

[1]
(c)

Calculate the volume of water taken up in this time, using volume = πr²l.

[2]
(d)

Explain why the shoot is cut under water when setting up the potometer.

[1]
(e)

A fan is switched on near the shoot. Predict and explain the effect on the bubble.

[3]
(f)

State two other environmental factors that increase the rate of transpiration.

[2]
(g)

Explain why a potometer measures water uptake rather than transpiration rate.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)loss of water vapour from leaves; evaporation from mesophyll surfaces then diffusion out through stomata
loss of water vapour from the leaves B1
by evaporation from the surfaces of mesophyll cells and diffusion through the stomata B1
2
(b)7.50 mm/min
37.5/5 = 7.50 mm/min B1
1
(c)29.5 mm³
π × 0.50² × 37.5 M1
29.5 mm³ A1
2
(d)to stop air entering the xylem
to prevent air bubbles entering / blocking the xylem B1
1
(e)moves faster; wind removes water vapour, steeper concentration gradient, more diffusion
bubble moves faster B1
moving air removes water vapour from around the leaf B1
maintaining a steep concentration / diffusion gradient so more water vapour diffuses out B1
3
(f)higher temperature; lower humidity; higher light intensity
Any 2 of: higher temperature; lower humidity; higher light intensity (stomata open) B2
2
(g)some water used in photosynthesis / kept in cells
some of the water taken up is used in photosynthesis / to keep cells turgid B1
so uptake is not exactly equal to the water lost B1
2
Question 6Structured · 14 marks

A student uses a potometer to measure water uptake by a leafy shoot. The distance moved by an air bubble in 5 minutes is recorded under three conditions. Table 6.1 shows the results.

conditionstill air, 20 °Cmoving air (fan), 20 °Cstill air, high humidity, 20 °C
distance moved in 5 min / mm20429
Table 6.1
(a)

Calculate the rate of water uptake in still air, in mm per minute.

[1]
(b)

The capillary tube has a cross-sectional area of 0.80 mm². Calculate the volume of water taken up per minute in moving air.

[2]
(c)

Explain why water uptake is greater in moving air.

[2]
(d)

Explain why water uptake is lowest in high humidity.

[2]
(e)

A potometer measures water uptake, not transpiration. Suggest why these are not exactly the same.

[1]
(f)

State two precautions needed when setting up a potometer, and explain one of them.

[3]
(g)

Describe the pathway of water from the soil to the air around the leaf.

[3]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)4.0 mm/min
20 ÷ 5 = 4.0 B1
1
(b)6.72 mm³ per minute
42/5 = 8.40 mm per min C1
× 0.80 = 6.72 mm³ per min A1
2
(c)water vapour blown away; steeper concentration gradient
water vapour is removed from around the leaf B1
maintaining a steep water vapour concentration (diffusion) gradient so more water evaporates / diffuses out of stomata B1
2
(d)air already moist; gradient shallow; less diffusion
air outside the leaf already contains much water vapour B1
so the concentration gradient is smaller and less water vapour diffuses out B1
2
(e)some water used in photosynthesis / kept in cells
some water taken up is used in photosynthesis or to keep cells turgid B1
1
(f)cut shoot under water; seal joints with vaseline; dry leaves
two precautions from: cut the shoot under water / joints sealed or airtight / leaves dry / leave the shoot to adjust before reading B2
one correct explanation, e.g. stops air entering the xylem and blocking water flow / prevents leaks B1
3
(g)root hair, cortex, xylem, mesophyll, evaporates, stomata
Any 3 of: enters root hair cells by osmosis; moves through cortex to xylem; up the xylem in the stem to the leaf; evaporates from mesophyll cells; diffuses out through stomata B3
3

More Transport questions

Want a mentor, not just a paper?

Riyaz Shaikh has mentored IGCSE, IB and A Level students for 15+ years, one-on-one, online and in Mumbai. The hardest questions on this site are the ones he uses to find a student's weak points.

Meet Riyaz →

Original Cambridge-style practice questions written for mentorriyaz.in. These are not Cambridge past paper questions, and this site is not affiliated with or endorsed by Cambridge University Press & Assessment. Updated 2026-11-04. Paper generator · mentorriyaz.in