Chromatography: IGCSE Chemistry questions with answers
Free Chromatography questions for IGCSE Chemistry (0620), part of Experimental techniques & analysis. Each question has the answer and the marking points an examiner would look for.
Two dyes, M and N, are separated by paper chromatography using water as the solvent. The solvent front moves 9.0 cm from the baseline. M moves 3.0 cm and N moves 7.0 cm. What is the Rf value of M and which dye is more soluble in water?
Rf value of M
more soluble dye
A
0.33
M
B
0.33
N
C
3.00
N
D
3.00
M
Show answer
Answer: B
Question 2Multiple choice · 1 mark
Fig. shows a chromatogram of dyes A, B, C and D and mixture X. Which dyes are in X?
Fig. 2.1
A B and D
B A and B
C C and D
D A and C
Show answer
Answer: C
Question 3Multiple choice · 1 mark
Why is the baseline in chromatography drawn in pencil?
A Pencil does not dissolve in the solvent.
B Pencil moves with the solvent.
C Pencil is easier to see.
D Ink is too dark.
Show answer
Answer: A
Question 4Structured · 4 marksA* challenge
A chromatogram of a food colouring shows three spots. A known dye E102 is run alongside.
(a)
State how the chromatogram shows that the food colouring is a mixture.
[1]
(b)
Describe how the chromatogram shows whether E102 is present.
[1]
(c)
Explain how colourless amino acids can be seen on a chromatogram.
[2]
Show answers and mark scheme
Part
Answer and marking points
Marks
(a)
more than one spot
it gives more than one spot B1
1
(b)
spot at same height / same Rf
a spot in the food colouring is at the same height (same Rf) as E102 B1
1
(c)
spray with locating agent
spray with a locating agent (ninhydrin) B1 and dry/heat to show spots B1
2
Question 5Structured · 3 marks
Chromatography can be used to separate amino acids.
(a)
Explain why the chromatography paper is put in a covered tank.
[1]
(b)
Explain why different substances travel different distances.
[2]
Show answers and mark scheme
Part
Answer and marking points
Marks
(a)
saturate air with solvent vapour
to stop the solvent evaporating B1
1
(b)
different solubility and attraction to paper
they have different solubilities in the solvent B1 and different attraction to the paper B1
2
Question 6Structured · 4 marks
Paper chromatography is used to identify the dyes in an ink.
(a)
Explain why the start line is drawn in pencil and not ink.
[1]
(b)
Explain why the start line must be above the solvent level.
[1]
(c)
A dye travels 3.6 cm and the solvent front travels 9.0 cm. Calculate the Rf value.
[2]
Show answers and mark scheme
Part
Answer and marking points
Marks
(a)
pencil insoluble, does not run
pencil (graphite) does not dissolve in the solvent / will not run B1
1
(b)
spots would dissolve into the solvent
otherwise the dyes would dissolve into the solvent in the beaker B1
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Original Cambridge-style practice questions written for mentorriyaz.in. These are not Cambridge past paper questions, and this site is not affiliated with or endorsed by Cambridge University Press & Assessment. Updated 2026-11-04. Paper generator · mentorriyaz.in