IGCSE · Chemistry 0620 · Experimental techniques & analysis

Chromatography: IGCSE Chemistry questions with answers

Free Chromatography questions for IGCSE Chemistry (0620), part of Experimental techniques & analysis. Each question has the answer and the marking points an examiner would look for.

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Chromatography practice questions

Question 1Multiple choice · 1 markA* challenge

Two dyes, M and N, are separated by paper chromatography using water as the solvent. The solvent front moves 9.0 cm from the baseline. M moves 3.0 cm and N moves 7.0 cm.
What is the Rf value of M and which dye is more soluble in water?

Rf value of Mmore soluble dye
A0.33M
B0.33N
C3.00N
D3.00M
Show answer

Answer: B

Question 2Multiple choice · 1 mark

Fig. shows a chromatogram of dyes A, B, C and D and mixture X. Which dyes are in X?

start linesolvent frontABCDX012345678cm
Fig. 2.1
  1. A B and D
  2. B A and B
  3. C C and D
  4. D A and C
Show answer

Answer: C

Question 3Multiple choice · 1 mark

Why is the baseline in chromatography drawn in pencil?

  1. A Pencil does not dissolve in the solvent.
  2. B Pencil moves with the solvent.
  3. C Pencil is easier to see.
  4. D Ink is too dark.
Show answer

Answer: A

Question 4Structured · 4 marksA* challenge

A chromatogram of a food colouring shows three spots. A known dye E102 is run alongside.

(a)

State how the chromatogram shows that the food colouring is a mixture.

[1]
(b)

Describe how the chromatogram shows whether E102 is present.

[1]
(c)

Explain how colourless amino acids can be seen on a chromatogram.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)more than one spot
it gives more than one spot B1
1
(b)spot at same height / same Rf
a spot in the food colouring is at the same height (same Rf) as E102 B1
1
(c)spray with locating agent
spray with a locating agent (ninhydrin) B1
and dry/heat to show spots B1
2
Question 5Structured · 3 marks

Chromatography can be used to separate amino acids.

(a)

Explain why the chromatography paper is put in a covered tank.

[1]
(b)

Explain why different substances travel different distances.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)saturate air with solvent vapour
to stop the solvent evaporating B1
1
(b)different solubility and attraction to paper
they have different solubilities in the solvent B1
and different attraction to the paper B1
2
Question 6Structured · 4 marks

Paper chromatography is used to identify the dyes in an ink.

(a)

Explain why the start line is drawn in pencil and not ink.

[1]
(b)

Explain why the start line must be above the solvent level.

[1]
(c)

A dye travels 3.6 cm and the solvent front travels 9.0 cm. Calculate the Rf value.

[2]
Show answers and mark scheme
PartAnswer and marking pointsMarks
(a)pencil insoluble, does not run
pencil (graphite) does not dissolve in the solvent / will not run B1
1
(b)spots would dissolve into the solvent
otherwise the dyes would dissolve into the solvent in the beaker B1
1
(c)0.40
Rf = 3.6 ÷ 9.0 C1
0.40 A1
2

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Original Cambridge-style practice questions written for mentorriyaz.in. These are not Cambridge past paper questions, and this site is not affiliated with or endorsed by Cambridge University Press & Assessment. Updated 2026-11-04. Paper generator · mentorriyaz.in